Figure 5: Scatter plot based on the matrix of the distances between full-length 16S rRNA sequences from the SILVA database closest to the representative sequences of OTUs found in V2-V3 and V3-V4 analyses.
From: The effect of 16S rRNA region choice on bacterial community metabarcoding results

The blue ellipse includes all OTUs detected using V3-V4 fragment analysis, while a red one includes all V2-V3 OTUs.