Figure 2
From: G-protein-coupled receptors mediate 14-3-3 signal transduction
Concentration–response curves of GPCR-mediated 14-3-3 signaling with various GPCRs. (a) Concentration–response curve of α1A-TEV/14-3-3ɛ-pLuc stably transfected cells treated with the α1A agonist cirazoline. (b) Concentration–response curve of ADORA2A-TEV/14-3-3ɛ-pLuc stably transfected cells treated with the adenosine receptor agonist NECA. (c) Concentration–response curve of DRD2-TEV/14-3-3ɛ-pLuc stably transfected cells treated with dopamine. (d) Concentration–response curve of CHRM3-TEV/14-3-3ɛ-pLuc stably transfected cells treated with the muscarinic acetylcholine receptor agonist carbachol. (e) Concentration–response curve of CHRM5-TEV/14-3-3ɛ-pLuc stably transfected cells treated with a panel of muscarinic acetylcholine receptor agonists: oxotremorine, pilocarpine and carbachol. (f) Concentration–response curves of CHRM5-TEV/14-3-3ɛ-pLuc stably transfected cells treated with the muscarinic acetylcholine receptor antagonists scopolamine and atropine and then stimulated with the agonist carbachol (3 μm). RLUs, relative light units.
