Table 2 TP ODN melting temperatures

From: Peptide-substituted oligonucleotide synthesis and non-toxic, passive cell delivery

ODN

Duplex

Modifications

T m

ΔT m

ΔT m per mod

ctrl

x′/x

0

70.0

—

—

4K

4K/x

4

63.0

−7.0

−1.75

6K

6K/x

6

56.6

−13.4

−2.23

4GR

4GR/x

4

66.3

−3.7

−0.93

6GR

6GR/x

6

59.0

−11.0

−1.83

2PA

2PA/x

2

66.6

−3.4

−1.70

3PA

3PA/x

3

67.6

−2.4

−0.80

2PG

2PG/x

2

54.6

−15.4

−7.70

3PG

3PG/x

3

64.3

−5.7

−1.90

2PJ

2PJ/x

2

61.0

−9.0

−4.50

3PJ

3PJ/x

3

62.0

−8.0

−2.67

  1. Abbreviations: mod, modification; TP ODN, triazolylphosphonate oligodeoxynucleotide; x, 5′-TAGCAGCACATCATGGTTTACA-3′; x′, 5′-TGTAAACCATGATGTGCTGCTA-3′.
  2. The TP ODN sequences are defined in Table 1. All Tms represent an average of at least three experiments; ΔTm is the difference in Tm when compared with Tm of the unmodified duplex.
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