Figure 7
Human ES2 ovarian cancer TIC PD-L1 alters rapamycin (rapa) and interferon (IFN)-γ sensitivity. (a) Fold change in numbers of ES2 TICs and non-TICs treated with 0.5 ng ml−1 IFN-γ for 60 h, versus medium alone. P-values, unpaired t-test. Absolute numbers of TICs (b) and non-TICs (c). Fold change in numbers of ES2 TICs (d) and non-TICs treated similarly with 5 nm rapamycin for 60 h, versus medium alone. Absolute numbers of TICs (e) and non-TICs (f). (g) Fold change in RPTOR expression by quantitative PCR. Each bar represents fold increase in gene expression in TICs versus respective total cultures, with P-value for same directly over bar. P-values on horizontal lines compare fold changes between ctrl and PD-L1lo cultures. All P-values, unpaired t-test.
