Figure 5
From: Platelets prime hematopoietic–vascular niche to drive angiocrine-mediated liver regeneration
SDF-1 activates CXCR7 on LSEC, inducing Akt-dependent activation of Id1 angiocrine pathway. (a) CXCR7 expression is essential for SDF-1-mediated Id1 nuclear induction. Early passages of primary human factor VIII+ LSECs (hLSECs) were stimulated with 10 ng ml−1 of SDF-1. shRNA silencing of Cxcr7 in human LSEC abolished nuclear accumulation of Id1 after SDF-1 treatment. Note SDF-1-induced translocation of Id1 from the cytoplasm to nuclei (arrow head). Scale bar, 10 μm. (b) PI3 kinase inhibitor Wortmannin blocked SDF-1-mediated upregulation of Id1, implicating that SDF-1 induction of Id1 is Akt-dependent. N=3–5 independent experiments.
