Figure 2

Effect of CIP2A expression on proliferation of MM cells. (a) MM.1S cells were transfected with a CIP2A expression plasmid, and total RNA was isolated 24 h after transfection and then subjected to reverse transcription PCR (RT-PCR) analysis, total protein was isolated and then subjected to western blot analysis. (b) 8226 cells were transfected with a CIP2A expression plasmid, and total RNA was isolated 24 h after transfection and then subjected to RT-PCR analysis, total protein was isolated and then subjected to western blot analysis. (c) MM.1S cells were transfected with a CIP2A expression plasmid, and then MTT was used to detect proliferation 24 h after transfection. (d) 8226 cells were transfected with a CIP2A expression plasmid, and then MTT was used to detect proliferation 24 h after transfection. (e) MM.1R cells were transfected with a CIP2A siRNA, and total protein was isolated 48 h after transfection and then subjected to western blot analysis. (f) MM.1R cells were transfected with a CIP2A siRNA, and then the number of viable cells was counted using a hemoatocytometer 48 h after transfection. (g) U266 cells were transfected with a CIP2A siRNA, and total protein was isolated 48 h after transfection and then subjected to western blot analysis. (h) U266 cells were transfected with a CIP2A siRNA, and then the number of viable cells was counted using a hemoatocytometer 48 h after transfection. (i) MM.1S or MM.1R cells were lysed and then subjected to western blot analysis. (j) MM.1R or U266 cells were transfected with a CIP2A siRNA, and total protein was isolated 48 h after transfection and then subjected to western blot analysis. (k) MM.1S or 8226 cells were transfected with CIP2A expression plasmid, and total protein was isolated 48 h after transfection and then subjected to western blot analysis. *P<0.05.