Figure 5

CIP2A promotes tumorigenesis in vivo. (a) Western blot of CIP2A from U266 cell lines, CIP2A^KD-U266, 8226 and CIP2A^OE-8226 cell lines selected with puromycin for 3 weeks after transfection. (b) Images of xenograft tumors obtained from mice. (c) CIP2A knockdown significantly inhibited MM tumor growth (CIP2A^KD-U266 group versus U266 group). (d) CIP2A overexpression significantly promoted MM tumor growth (CIP2A^OE-8226 group versus 8226 group). (e) Survival curve of CIP2A^KD-U266 and U266 group mice. (f, g) Weight of the tumor from each group taken out from the mice being killed at the end of the study. (h) Tumor tissues were collected from mice; whole-tissue lysates were subjected to western blot using indicated antibodies. (i) Tumor tissues were collected from mice, and PP2A activity was measured by PP2A phosphatase assay. *P<0.05, **P<0.01.