Figure 6

Immunofluorescence of tumor and primary organs samples after injection of trastuzumab, T-SA1 and T-SA2 (scale bars, 50 μm).Trastuzumab, T-SA1 and T-SA2 were detected in red fluorescence and cell nuclei were detected in blue fluorescence. (a) The same as trastuzumab, T-SA1 and T-SA2 distributed in tumor, liver and kidney tissues at 12 h post injection. Rather than appeared diffuse distribution in intercellular substance of liver and kidney tissues, antibodies accumulated in the cytoplasm in entire tumor tissues. (b) The fluorescence intensity of antibodies in tumor tissues increased with time. Among the three antibodies, T-SA1 had the widest distribution in tumor tissues.