Figure 5

Attenuated gastric acid secretion in QS11-treated mice. (a) Wild-type (WT) mice were divided into three groups and consistently subjected to 20 μm QS11, 40 μm QS11 or dimethyl sulfoxide (DMSO) as control for 7 days. After 30 min of histamine stimulation, the mice were killed and gastric tissues from three independent individuals in each group were evaluated for the expression of total Arf6, GTP-Arf6. The GAPDH blotting served as loading control. (b) Following intraperitoneal injection of 20 μm QS11, 40 μm QS11 or DMSO for 7 days, the histamine-stimulated gastric fluid pH in the three groups was measured respectively. QS11 elicited dose-dependent effect on the inhibition of histamine-stimulated gastric acid secretion as compared to DMSO as control (P-value indicated in the figure, Student’s t-test). The uneven effects of QS11 administration might be due to the variable levels of total Arf6 in different individuals.