Figure 4
From: Ageing affects chondroitin sulfates and their synthetic enzymes in the intervertebral disc

(1) Representative images of AF tissue stained with (a–c) Safranin O/FCF; (d–f) for XT-I and (g–i) for GT-I in 6-month-old (a, d and g), two year-old (b, e and h), and 8-year-old (c, f and i) bovine IVD tissues (n=5; scale bar=100 μm). (2) Quantification of XT-I and GT-I staining intensities in AF tissue. Data were normalized to the surface area and are represented as the mean±standard error of the mean (n=5). * denotes significant differences between the different groups at P<0.05.