Table 2 Primers for constructing JEV-infectious DNA clone
Primers | Sequences |
|---|---|
F1 | 5′- TTGGCGCGCCATTTAGGTGACACTATAGAGAAGTTTATCTGTGTGAACTTCTT-3′, AscI sp6 promoter |
R1 | 5′- CCTGG C GCCTGCACAAGCTATGACAAC-3′, KasI |
F2 | 5′- TTGGCGCGCCAATGCAGG C GCCATGAAGTTGTCGAAT-3′, AscI, KasI |
R3 | 5′- GGTCCGGACCAGTCTAGTGACAGATCTGACTC-3′, BspEI, BglII |
F4 | 5′- TGCGGAGTCAGATCTGTCACT-3′, BglII |
R4 | 5′- CATTTTCTGTCCGGAATCGTAG-3′, BspEI |
F5 | 5′- CGCCCCTACGATTCCGGACAGA-3′, BspEI |
R6 | 5′- GGAAAAGGATCCGTGGTTCCAGG-3′, BamHI |
F7 | 5′- ACCACGGATCCTTTTCCTGACTC-3′, BamHI |
R8 | 5′- CCGCTCGAGCGGTGCTCTAGAACGCGTGTATTCCGACGTGATCAGGTG-3′, XhoI, XbaI, MluI, BclI |
F9 | 5′- CACCTGATCACGTCGGAATA-3′, BclI |
R10 | 5′- CCGCTCGAGCCCAAAGCTTCAAACTCTAGAT-3′, XhoI, XbaI |
F11 | 5′- GCTTGGAGCACGGTATCTAGAGTT -3′, XbaI |
R11 | 5′- CCGCTCGAGAGATCCTGTGTTCTTCCTCACCAC-3′, XhoI |
Serial nos | Mutations | SA14-14 | SA14-14-2 |
|---|---|---|---|
1 | E-107 | Phenylalanine | Leucine |
2 | E-138 | Lysine | Phenylalanine |
3 | E-176 | Valine | Isoleucine |
4 | E-177 | Alanine | Threonine |
5 | E-264 | Methionine | Lysine |
6 | E-279 | Valine | Alanine |
7 | E-315 | Proline | Serine |
