Table 3 Primers used to generate JEV mutations
Primers | Sequences | Mutations |
|---|---|---|
177-F | 5′- CCAATGCTCCTTCGGTAACCCTCAAAC-3′ | G to A |
177-R | 5′- GTTTGAGGGTTACCGAAGGAGCATTGG-3′ | C to T |
264-F | 5′- GAAGGAGGCCTCCATCAGGCGTTG-3′ | T to G |
264-R | 5′- CAACGCCTGATGGAGGCCTCCTTC-3′ | A to C |
279-F | 5′ - GTACTCAAGCTCAGTGAAGTTAACATCAGG-3′ | T to A |
279-R | 5′- CCTGATGTTAACTTCACTGAGCTTGAGTAC-3′ | A to T |
315-F | 5′- GAAAAATCCGGCGGACACTGGTCAC-3′ | T to C |
315-R | 5′- GTGACCAGTGTCCGCCGGATTTTTC-3′ | A to G |
F | 5′- TTGGCGCGCCAATGCAGG C GCCATGAAGTTGTCGAAT-3′, AscI, KasI | A to C silent mutation |
R | 5′- GGTCCGGACCAGTCTAGTGACAGATCTGACTC-3′, BspEI, BglII |
