Figure 4
From: Fibromodulin reduces scar formation in adult cutaneous wounds by eliciting a fetal-like phenotype
FMOD enhanced TGFβ1-induced rat dermal fibroblast (RDF) migration and invasion, and myofibroblast conversion and contraction. FMOD+TGFβ1 significantly promoted TGFβ1-mediated RDF migration (a) and invasion through collagen matrices (b). Flow cytometry tests revealed that FMOD significantly enhanced TGFβ1-induced rat dermal fibroblast-myofibroblast conversion (c), which corresponds with immunofluorescent staining (d). Interestingly, after 48 h treatment, α-Sma+ myofibroblasts formed stress fibers (yellow arrows) in the FMOD+TGFβ1 group. Additionally, FMOD+TGFβ1 significantly stimulated TGFβ1-mediated myofibroblast contraction in collagen gel (e). Dosages: TGFβ1 (100 pm) and FMOD (200 nM). Scale bar=100 μm (a, b), or 50 μm (d). Two-sample t-test was used for statistical analysis, and data are shown as mean±the standard deviation N=4 (a, b), 3 (c), or 6 (e). *P<0.05, **P<0.01.
