Figure 2
From: STAT3 mediates C6-ceramide-induced cell death in chronic lymphocytic leukemia

CNL suppresses the phosphorylation of STAT3 at both Y705 and S727 residues. CNL suppresses phosphorylation of STAT3 in (a) CLL patient cells; (b) JVM-3 cells; (c) Mec-2 cells; (d ) ex vivo xenograft tumors. Cells were treated with 20 μM and/or 40 μM of ghost nanoliposomes or CNL as indicated in the figure for 24 h (CLL patient cells and JVM-3 cells) or 48 and 72 h (Mec-2 cells). Western blotting analysis was performed. The graphs represent the quantification of western blotting from: (a) 7 CLL patient cells; and (b) three independent experiments. The final western blot image was created by grouping different parts of the same film of the same gel as indicated by the black dividing line. Statistical analysis was performed using Student’s t-test, *P<0.05, **P<0.01. (d) JVM-3 xenograft tumors were obtained from a subcutaneous CLL mouse model in Balb/c Nu/nu mice that were injected with ghost nanoliposomes or CNL (from Ryland et al.13). Western blotting was performed for one tumor treated with ghost nanoliposomes and two CNL-treated tumors obtained from two separate mice. (e) CNL does affect cell viability and STAT3 phosphorylation in HEK293 cells. (i) Cell viability of HEK293 cells was determined by MTS assay after 24 h treatment and (ii) western blotting analysis was performed. The results are representative of three independent experiments.