Figure 7
From: STAT3 mediates C6-ceramide-induced cell death in chronic lymphocytic leukemia
Overexpression of STAT3-C rescues CNL-induced cell death. (a) STAT3-C-expressing cells are resistant to CNL-induced cell death. Lentiviral transduction was performed to express STAT3-C in JVM-3 cells. Seventy-two hours after the last transduction, FACS was performed to obtain a pure population of cells expressing STAT3-C and the treatments were done. An overexpression construct expressing RFP was used as a negative control. Seventy-two hours after the last transduction cycle, cells were treated with ghost liposomes and CNL for 24 h. (i) Expression of STAT3-C was confirmed by western blotting and probing for Flag-tag. (ii) Flow cytometric analysis for Annexin-V and 7AAD was performed to quantitate % necrotic cells. Student’s t-test was used for statistical analysis, *P<0.05.
