Figure 2

Mice with myeloid-specific knockout of MCPIP1 are susceptible to LPS-induced inflammation and lung injury. (a) Serum levels of proinflammatory cytokines and chemokines in Mcpip1^flfl and M-Mcpip1^−/− mic, treated intraperitoneally with PBS or LPS for 8 h. Data are presented as the means±s.d. (n=5). *P<0.05, versus Mcpip1^flfl group. (b) H&E staining of lung sections from Mcpip1^flfl and M-Mcpip1^−/− mice at 2 months of age treated intraperitoneally with PBS or LPS for 8 h. Original magnification is 100×. Data are representative of three independent experiments. (c) Immunohistological staining of lung sections from Mcpip1^flfl and M-Mcpip1^−/− mice at 2 months of age treated intraperitoneally with PBS or LPS for 8 h, with anti-F4/80 or Gr-1 antibodies. Original magnification is 100× or 400×, as indicated. Data are representative of three independent experiments. (d) Changes of lung wet-to-dry ratios in Mcpip1^flfl and M-Mcpip1^−/− mice treated intraperitoneally with PBS or LPS for 8 h. Data are presented as the means±s.d., *P<0.05 by Student’s t-test.