Figure 5

SiRNA-mediated knockdown of MALT1 selectively increased the abundance of the MCPIP1 protein and suppressed LPS-induced macrophage activation. (a) Raw264.7 cells were transfected with si-Control or si-MALT1 for 24 h. Transfected cells were stimulated with or without 100 ng/ml LPS for 8 h. The protein levels of different genes were determined by immunoblot with individual antibodies as indicated. β-actin served as a loading control. (b) Fold-change of the MCPIP1 and C/EBPδ protein levels were determined by densitometry and normalized to β-actin. Data are presented as the means±s.d. (n=3), *P<0.05 by Student’s t-test. (c) Raw264.7 cells were transfected with si-Control or si-MALT1 for 24 h. Transfected cells were stimulated with or without 100 ng/ml LPS for 8 h. The mRNA levels of MCPIP1 and cytokines as indicated were determined by qPCR analysis and normalized to actin mRNA levels. Data are presented as the means±s.d. (n=4), *P<0.05 by Student’s t-test.