Figure 6

Administration of MI-2 prevented LPS-induced MCPIP1 protein degradation in vivo. (a) Adult C57BL/6 mice were intraperitoneally injected with LPS (25 mg/kg body weight) for different times as indicated. The mice were euthanized, and the lungs were collected for immunoblot analysis with different antibodies, as indicated. β-actin serves as a loading control. (b) Fold-change of the protein levels were determined by densitometry and normalized to β-actin. Data are presented as the means±s.d. (n=3). (c) Adult C57BL/6 mice were intraperitoneally injected with LPS (25 mg/kg body weight) for 8 h. Mice were euthanized, and the lungs were collected for immunoblot analysis with specific antibodies, as indicated. β-actin serves as a loading control. (d) Fold-changes of the protein levels were determined by densitometry and normalized to β-actin. Data are presented as the means±s.d. (n=3). (e) Adult C57BL/6 mice were intraperitoneally injected with PBS or LPS (25 mg/kg body weight) for 8 h. One group of mice was pretreated with MI-2 for 2 h and then injected with LPS for 8 h; the other group of mice was injected with LPS for 2 h and then treated with MI-2. Mice were euthanized 8 h after LPS injection. The lungs were collected for immunoblot analysis with specific antibodies as indicated. β-actin serves as a loading control. (f) Fold-changes of protein levels in (e) were determined by densitometry and normalized to β-actin. Data are presented as the means±s.d. (n=3). *P<0.05, **P<0.01 by Student’s t-test.