Figure 2

Frequency of WBD infections when sterile gauze containing 5 ml of the different experimental treatments was attached to healthy A. cervicornis fragments.

WBD homogenate was airbrushed coral with active WBD, 0.45 μM was the filtrate that passed through a 0.45 μM filter and 0.22 μM the filtrate that passed through a 0.22 μM filter. The tetracycline treatment was active WBD homogenate that was treated with 100 μg/ml Tetracycline with 20 μg/ml imidocarb diproprionate for two hours prior to attachment, the Ampicillin treatment was treated with 100 μg/ml for two hours and the controls were 5 ml of airbrushed tissue from healthy A. cervicornis colonies. Disease progression was scored two days after attachment of treatments and treatments were scored as transmitting WBD if there was more than 1 cm of dead tissue that progressed along the coral branch over time. In this figure treatments with (a) were statistically different from the controls (p<0.0001, Fisher-exact tests with Bonferroni adjustments) and those with (b) were not (p>0.1, Fisher-exact tests with Bonferroni adjustments).