Figure 2
From: Generation of stable Drosophila cell lines using multicistronic vectors
pAc5-STABLE1-Neo confers G418 resistance to S2R+ cells.
(A–I) Bright field images of S2R+ Drosophila cells that were mock-transfected (Ctrl; A–C), transfected with pAc5-GFP (D–F) or with pAc5-STABLE1-Neo (G–I). Three days after transfection, G418 was added to the media at concentrations of 0 (A, D, G), 600 (B, E, H) or 2000 μg/ml (C, F, I). (A'–I') Fluorescent images of the same cells from panels A to I showing GFP expression. (J–L) Percentage of surviving cells that are positive for GFP. Letters correspond to panels/treatments directly above each graph. All images were taken after 30 days of treatment with G418.
