Figure 3
From: Generation of stable Drosophila cell lines using multicistronic vectors
T2A is correctly processed in Drosophila cultured cells.
Western blot analysis of S2R+ cells transfected either with pAc5-STABLE1-Neo or pAc5-GFP vectors, probed with anti-GFP antibodies. Expected sized of non-processed GFP-T2A-neo is shown (58kDa; arrow). The slight difference in size between the GFP proteins derived from the two plasmids is caused by additional polylinker-derived amino acids in the case of pAc5-GFP. Molecular weights in kDa are indicated to the left.
