Figure 5
From: Generation of stable Drosophila cell lines using multicistronic vectors
Expression of two different proteins as well as a selectable marker from a single vector in Drosophila cultured cells.
(A–I) Brightfield images of Drosophila S2R+ cells that were mock-transfected (Ctrl; A–C), transfected with pAc5-GFP (D–F) or transfected with pAc5-STABLE2-Neo (G–I). Three days after transfection, G418 was added to the media at concentrations of 0 (A, D, G), 600 (B, E, H) or 2000 μg/ml (C, F, I). Fluorescent images of the same cells from panels A–I showing GFP expression (A'–I') or FLAG-mCherry expression (A”–I”). White boxes mark zoomed region shown in inset (panel I, overlay). All images were taken after 30 days of treatment with G418.
