Figure 6
From: Generation of stable Drosophila cell lines using multicistronic vectors
T2A and dT2A are correctly processed in Drosophila cultured cells.
Western blot analysis of S2R+ cells transfected either with pAc5-STABLE1-Neo or pAc5-STABLE2-Neo vectors, or only mock-transfected. Observed bands using antibodies against GFP and FLAG (to detect FLAG-Cherry) were consistent with correct processing of the T2A/dT2A sequences. Expected sizes of non-processed FLAG-Cherry-dT2A-GFP-T2A-neo (91kDa; white arrow) or GFP-T2A-neo (58kDa; black arrow) are depicted; none is detected in either case, even in long exposures. Molecular weights in kDa are indicated to the left.
