Figure 7
From: Generation of stable Drosophila cell lines using multicistronic vectors
dTes-GFP colocalises with actin cytoskeleton in S2R+ cells.
(A–D) Confocal pictures showing the expression of GFP encoded by pAc5-STABLE1-Neo (GFP-T2A-neo; A, B) or the fusion protein encoded by pAc5–dTes.GFP-Neo (dTES-GFP; C, D). Cells are stained with Alexa568-Phalloidin (red) to show the F-actin cytoskeleton and with DAPI (blue) to show the nuclei. Grayscale images represent single green (A'–D') or single red channels (A”–D”). B and D show the region indicated in A or C, respectively. Arrowheads in B and D indicate dTes-GFP protein accumulation, while arrows show F-actin. Scale bars in A and C = 8 μm. (E) Western blot analysis of S2R+ cells transfected with pAc5-dTES.GFP-Neo and probed with anti-GFP. A single band of expected size is observed. Molecular weights in kDa are indicated to the left.
