Figure 1
From: A rapid and robust tri-color flow cytometry assay for monitoring malaria parasite development
Flow cytometry gating strategy for P. yoelii parasitemia determination.
The TCM was performed with blood from (A) an uninfected C57BL/6J mouse and (B) a mouse infected 5 days previously with P. yoelii 17X clone 1.1. Red blood cells and white blood cells are first gated (G1) on a forward scatter/side scatter (FSC-A/SSC-A) dot plot. The G1 events are visualized using a FSC-A/FSC-H dot plot and the singlets (single cells) are gated on gate G2. Cells on G2 are simultaneously displayed on both CD45/Hoechst and Ethidium/Hoechst dot plots. CD45-positive white blood cells (WBCs) appear in gate G3 and are subtracted from all DNA- and RNA-containing cells (gate G4) to calculate the parasitemia.
