Figure 2
From: A rapid and robust tri-color flow cytometry assay for monitoring malaria parasite development
Flow cytometry gating for P. berghei ANKA parasitemia determination.
The TCM method was performed with blood from (A) an uninfected C57BL/6J mouse and (B) a mouse infected 7 days previously with P. berghei ANKA expressing GFP. The same flow cytometry gating strategy described in Figure 1 was used. On the extreme right panel, GFP expression for cells present in gate G4 was displayed on the SSC/GFP dot plot. In gate G4, the parasite population in (1) is composed of a mix of rings and trophozoites and in (2) of a mix of multi-rings, schizonts and gametocytes as shown in representative Giemsa-stained smears obtained after sorting by flow cytometry.
