Figure 4
From: A rapid and robust tri-color flow cytometry assay for monitoring malaria parasite development
Monitoring of P. falciparum parasitemia grown in vitro by the TCM.
(A) Ethidium/Hoechst dot plot of parasites obtained from a synchronized culture of P. falciparum (3D7) after excluding debris and doublets. Cryopreserved parasites were thawed and stained by the TCM at two different times after thawing. The indicated cell populations were sorted by flow cytometry and stained with Giemsa; they corresponded to different parasite stages: (1) single ring, (2) double ring, (3) trophozoite, (4) early schizont and (5) late schizont. (B) Ethidium/Hoechst dot plot of parasites from an asynchronous culture of P. falciparum 3D7 before (left panel) and after (right panel) magnetic sorting using a MACS column. Predominantly, the stages containing the pigment (hemozoin) are in the Ethidiumhigh gate as shown in representative Giemsa-stained smears (right panel).
