Figure 2
From: Huntingtin mediates dendritic transport of β-actin mRNA in rat neurons
Knockdown of Htt decreases β-actin mRNA levels in rat neurons leading to accumulation in the soma.
(a) DIV 4 cortical neurons were infected with lentivirus expressing scrambled shRNA, shRNA-Htt-1, or shRNA-Htt-2 and probed at DIV 8 for β-actin mRNA (red), Htt (blue), GFP (green, with anti-GFP antibody). Representative images from each experiment are shown. The left part of each image is the proximal part of the dendrite. Scale bar: 5.0 μm. (b) Relative intensity of Htt and β-actin mRNA shown in (a), normalized to the area of GFP staining, which represents size of dendrite. The percent shown in parentheses indicates extent of knockdown for Htt and reduction in signal for β-actin mRNA relative to scrambled. (c) Representative images of β-actin mRNA and GFP in a whole neuron after knockdown of Htt. Scale bar: 10.0 μm. (d) Quantitative analysis of enrichment of β-actin mRNA in the soma shown in (c). Intensity of β-actin mRNA in the soma was divided by total β-actin mRNA. Control sample (infected with lentivirus carrying scrambled shRNA) was set to 1. n = 2.
