Table 1 Effect of knockdown on the expression of proteins in the transport machinery.

From: Huntingtin mediates dendritic transport of β-actin mRNA in rat neurons

 

Htt

HAP1

KIF5A

Dynein

shRNA-Htt-1

------

→

→

→

shRNA-HAP1

→

------

↓(16.4%) 0.84/5.13

↓(32.3%) 0.94/2.91

shRNA-KIF5A

↓(31.1%) 1.96/6.31

↓(35.2%) 1.60/4.54

------

↓(17.5%) 0.51/2.91

shRNA-DHC

→

↓ (33.3%) 1.51/4.54

↑ (160%) 8.20/5.13

------

  1. → No significant changes.
  2. The percentage was calculated as follows: normalized intensity (underlined index number) after knockdown of target protein with indicated shRNA, was divided by normalized intensity (italicized index number) of the same target with scrambled shRNA. The area of anti-GFP staining in 30 μm dendrites was used for normalization.
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