Figure 2
From: Tandem assembly of the epothilone biosynthetic gene cluster by in vitro site-specific recombination
Strategy for assembling the epothilone biosynthetic gene cluster by SSRTA.
(a) Map of seven ‘entry vectors’ used in this study. Arrows in pink, green, dark green, red, lime, orange and blue represent pairs of recombination sites numbered 0, 6, 13, 7, 12, 3 and 15, respectively. (b) Map of plasmid pZLE10. Details are available in the Online Methods and Supplementary Table 2. (c) Schematic of the tandem assembly of pZLE10-epoD. Five groups of recombination reactions occur simultaneously, between attB0/attP0 , attB6/attP6 , attB13/attP13 , attB3/attP3 and attB15/attP15 . (d) Schematic of pZLE10-epo assembly. Seven groups of recombination reactions take place, between attB0/attP0 , attB6/attP6 , attB13/attP13 , attB7/attP7 , attB12/attP12 , attB3/attP3 and attB15/attP15 .
