Figure 6 | Scientific Reports

Figure 6

From: Essential role of microfibrillar-associated protein 4 in human cutaneous homeostasis and in its photoprotection

Figure 6

MFAP-4 directly interacts with fibrillin-1 to form microfibrils.

(a) Concentrated supernatants from NHDFs cultured for 8 days were immunoprecipitated with an anti-MFAP-4 antibody or normal rabbit IgG. Immunoprecipitants were analyzed by Western blotting with an anti-fibrillin-1 antibody. (b) NHDFs were transfected with siRNAs for non-specific sequences (Control) or MFAP-4 twice during the culture for 8 days, followed by immunofluorescence staining with an anti-human MFAP-4 antibody (green) and an anti-human fibrillin-1 antibody (red). Nuclear staining (DAPI) and merged images are also shown. Scale bars = 50 μm.

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