Figure 1 | Scientific Reports

Figure 1

From: Single molecule tracking fluorescence microscopy in mitochondria reveals highly dynamic but confined movement of Tom40

Figure 1

Schematic diagram of the optical setup.

A wide-field yellow excitation laser beam is shuttered by an acousto-optical modulator, focused onto the back aperture of an Olympus TIRF objective, which collimates the laser light at an inclined angle. A violet photoconversion laser beam is shuttered by a mechanical shutter and is spatially overlapped with wide-field yellow imaging beam. An anamorphic prism pair (06 GPA 204, Melles Griot) corrects for asymmetry of this laser beam to produce a TEM00 mode.

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