Figure 4
Analysis of Tom40 diffusion as compared with immobilized Dendra2 molecules.
(A) One experimentally obtained single molecule trajectory for Tom40 with a frame time of 5 ms and an exposure time of 5 ms. (B) One experimentally obtained single molecule trajectory for immobilized Dendra2 with a frame time of 5 ms and an exposure time of 5 ms. (C) Trajectory-averaged mean square displacements (MSDs) in the sample plane of the coverslip over different time intervals. The error bars represent the experimental standard errors of the means. MSDs from Tom40 are displayed in red and MSDs from immobilized Dendra2 molecules are displayed in black. (D) Trajectory-averaged cumulative distribution functions (CDFs) of displacements over 10 ms in the sample plane of the coverslip for Tom40 (in red) and immobilized Dendra2 (in black). The error bars represent the experimental standard errors of the means. (E) Trajectory-averaged cumulative distribution functions (CDFs) of displacements over 15 ms in the sample plane of the coverslip for Tom40 (in red) and immobilized Dendra2 (in black). The error bars represent the experimental standard errors of the means. (F) Step-averaged cumulative distribution functions (CDFs) of displacements over 5–35 ms in the sample plane of the coverslip for Tom40 (solid lines) and immobilized Dendra2 (dashed lines), color-coded as indicated in the insert box.
