Figure 1
Generation of polyclonal antibodies against Ubx.
(A) Top: schematic of Ubx protein. N-terminal 240-aminoacid region was expressed for raising antibodies. Below: Western blot analysis to show specificity of the polyclonal antibodies raised against Ubx. See a single prominent band of full-length protein (arrows) detected in embryonal, haltere disc and CbxHm /+ wing disc lysates. S2 cells, T1 and T2 leg discs (which does not express Ubx) are used as negative control. Equal amount of protein was loaded in all the lanes. These antibodies were used to identify potential direct targets of Ubx. Validation of potential targets of Ubx. (B) Candidate regions were selected randomly from high, medium and low enrichment levels of ChIP-chip data and validated by two independent biological replicates of ChIP-qPCR for chromatin isolated from CbxHm/+ discs. Primers for qPCR were designed around the probes enriched in ChIP-chip. Bar height represent mean enrichment of test IP over mock IP and error bars represent deviation from the mean enrichment. Note, all 14 targets regions are enriched in both the replicates. Brackets next to gene name represent arbitrarily assigend names for multiple probes enriched for the same gene in ChIP-chip data. See Supplementary Material for details.
