Figure 1
From: A transgenic mouse model for monitoring oxidative stress
Schematic of OKD48 function.
p(3xARE)TKbasal-hNrf2(1-433)GL4-Flag was generated as the OKD48 construct. In human Nrf2, the Neh2 domain (red) functions as the Keap1 binding and ubiquitinated region and the Neh1 domain (blue) functions as the DNA binding region. The OKD48 construct has 3xARE promoter (light blue), human Nrf2 (a.a.1–433; purple) and Flag-tagged luciferase (GL4; yellow). Under normal conditions, transcription of the OKD48 construct was not induced and the leaked OKD48 protein was degraded by the Keap1 (orange) system. Upon oxidative stress, OKD48 was transcriptionally induced by the 3xARE element and the resulting protein was stabilised by the Keap1 system. Thus, we detected luminescence only in cells experiencing oxidative stress.
