Figure 6
From: A transgenic mouse model for monitoring oxidative stress
In vivo oxidative stress assay with OKD48 transgenic mice.
(a) Luminescence activity with several concentrations of ASN in internal organs. OKD48 transgenic mice were intraperitoneally injected with several concentrations of ASN. After 6 hr, the mice were abdominally operated and analysed using the in vivo imaging system. (b) Luminescence activity with several concentrations of ASN in liver. Livers in Fig. 6a were surgically eviscerated and analysed using the in vivo imaging system. (c) Induction of HO-1 mRNA with several concentrations in liver. Total RNAs from each ASN-injected mouse liver were subjected to quantitative PCR analysis. GAPDH was used as an internal standard. (d) Multiple detection of OKD48 signal in vivo. At day 1, OKD48 transgenic mice were intraperitoneally injected with ASN. After 6 hr, the mice were analysed using the in vivo imaging system. After an interval of 4 days (at day 5), the same individuals were re-injected with ASN and analysed similarly to day 1. (e) Luminescence activity with UV-A irradiation in vivo. Before (left) and after (right) UV-A irradiation (30 mW per cm2 (upper) and 5 mW per cm2 (lower)), OKD48 transgenic mice were analysed using the in vivo imaging system. (f) Induction of HO-1 mRNA with UV-A irradiation. Total RNAs from mouse skins irradiated with or without UV-A were subjected to quantitative PCR analysis. GAPDH was used as an internal standard.
