Figure 2
From: The precision of axon targeting of mouse olfactory sensory neurons requires the BACE1 protease
BACE1 IR predominantly colocalizes with pre-synaptic neuronal marker proteins in the mouse olfactory bulb.
(a – c) Colocalization of BACE1 IR and NCAM IR in glomerular layer (GL) but not in the outer nerver layer (ONL). (d) Percentage of overlapping pixels of NCAM and BACE1 in GL and ONL. (e – g) BACE1 IR overlaps with OMP IR, a specific marker of OSNs, in GL. (h) Percentage of overlapping pixels of OMP and BACE1. (i – k) BACE1 IR overlaps with GAD65 IR, a specific marker of inhibitory periglomerular cells in GL. (l) Percentage of overlapping pixels of of GAD65 and BACE1 revealed that it is higher in GL than that in external plexiform layer (EPL). (m – o) Colocalization of BACE1 IR with MAP2 (a dendritic marker protein) immunoreactivity in GL and OPL. (p) Quantification of the overlapping level of MAP2 and BACE1. (q – s) BACE1 colocalizes with postsynaptic protein PSD95 in GL and EPL. (t) Quantification of the overlapping level of PSD95 and BACE1. * = p < 0.05.
