Figure 1

Basal currents of missense variants identified in TRESK.

(A) Schematic topology of the human K2P TRESK subunit showing the position of the identified missense variants. (B) Side view of homology model of the TM/pore domains of TRESK with the A34 and C110 residues shown in blue and red respectively. For clarity only the P1 domain is shown. (C) Representative whole-cell currents from an oocyte expressing either WT TRESK or the variants. Oocytes were bathed in lowK solution, the membrane potential held at −80 mV and voltage steps from −120 to +60 mV applied for 1 sec in 20 mV increments. (D) Normalised basal currents for the TRESK variants. Steady state currents were measured at the end of the 1s pulse to +60 mV from the whole cell currents (as shown in C). For each batch of oocytes, the currents were normalised to the average of control group. Data were pooled from 2 to 4 batches. Oocytes were injected with 0.9 ng of RNA except for the C110R mutant where 4.5 ng was injected. N-numbers are given above the bars.