Figure 3
From: TNF-α and TGF-β Counter-Regulate PD-L1 Expression on Monocytes in Systemic Lupus Erythematosus
Expression of PD-L1 on isolated CD14 + cells is not entirely dependent on lymphocytes.
(A) and (C) Representative histograms demonstrate PD-L1 induction on total PBMC or isolated CD14+ cells from a control subject and an active SLE patient. Protein expression was assayed by flow cytometry after culturing cells for 24 hours without stimulation. (B) and (D) PD-L1 expression on monocytes gated from total PBMC compared to isolated monocytes and myeloid DCs in eight healthy controls and eight SLE patients. (E) and (F) Fold induction of PD-L1 MFI on isolated CD14+ SLE monocytes treated with TNF-α or with anti-TGF-β mAb. (G) and (H) Fold induction of PD-L1 MFI on isolated CD14+ monocytes from healthy donors treated with anti-TNF-α mAb or with recombinant TGF-β. Induction was quantified by dividing the MFI of cytokine-treated samples by that of untreated cells and tested for significance by the Wilcoxon signed-rank test.
