Figure 2
Investigation of real target organs of TW295-induced aggravation of colitis.
(a) Detection of infected bacteria in organs by PCR. Whole DNA was extracted and the nested PCR method was performed. Each organ represents the results from two different animals. (b) Confirmation of viable bacteria in liver tissue. The tissue specimens were cut into small pieces and squeezed, then inoculated onto mitis salivarais bacitracin (MSB) agar plates. (c) Time-dependent localizations of viable bacteria in liver tissues. Viable cell numbers are expressed as colony forming units (CFU)/mg tissue protein. Each point represents the mean ± SEM from 3–5 different animals. (d) Immunohistochemical stainig of serotype k bacteria in the DSS mouse liver. Arrowheads represent positive staining. (e) Localization of GFP-expressing TW295 in the mouse liver. Administered GFP-expressing TW295 were uptaken by hepatocytes, but not by endothelial cells. Left, low magnification of central vein area; right, high magnification of small blood vessel. The blue areas represent nuclear staining by 4’6-diamino-2-phenylindole (DAPI). CV, central vein; BV, blood vessel. (f) Uptake of TW295 by Kupffer cells using ex vivo electron microscopy. (g) Uptake of TW295 by hepatocytes using ex vivo electron microscopy. Arrowheads represent TW295 uptake by hepatocyte.
