Figure 4
TW295-mediated increase in IFN-γ productions and effect of anti-IFN-γ antibody on TW295-mediated aggravation of colitis.
(a): Increased expression of IFN-γ in liver tissues after TW295 to mice with DSS-induced colitis and normal controls. Arrow indicates the size of IFN-γ. GAPDH is an internal standard for PCR. (b): Immunohistochemical staining of IFN-γ in the liver and colon of mice after the administration of TW295 or vehicle (Control). The brown areas represent positive expression of IFN-γ. (c) & (d): Increased expression of IFN-γ on a hepatocarcinoma cell line, Huh-7 (c) or primary human hepatocytes (d) after the application of bacteria. Neg Con; negative control. GAPDH is an internal standard for PCR. (e): Effect of anti-IFN-γ antibody on DAI in TW295-infected DSS-induced colitis mice at 6 days after infection of TW295. The anti-IFN-γ polyclonal antibody (0.5 mg/mouse) was intraperitoneally administered mice at 4 and 24 hours after the TW295 infection. Each column represents the mean ± SEM from 10–18 different animals. Statistical significance was determined using Student’s t-test. **p = 0.0099. (f): Effect of anti-IFN-γ antibody on survival rate in TW295-infected DSS-induced colitis mice at 11 days after infection of TW295. The anti-IFN-γ polyclonal antibody (0.5 mg/mouse) was intraperitoneally administered mice at 4 and 24 hours after the TW295 infection. Each column represents the mean from 10–18 different animals. Statistical significance was determined using Student’s t-test. **p = 0.0059.
