Figure 2

CNS lymphocyte infiltrates do not differ between LMP2A transgenic mice and litter mate controls.

Mononuclear cells were isolated from spinal cords of LMP2A and litter mate control (WT) mice at pre-clinical, peak and recovery stages of disease. a, Flow cytometric gating strategy used to quantify total cells and lymphocyte subsets. b, Enumeration of infiltrating cells in LMP2A and WT littermates at all three stages of disease using trypan blue exclusion. c-e, Infiltrating cells were stained to identify CD45^hiCD19⁺, CD45^hiCD3⁺CD4⁺ and CD45^hiCD3⁺CD8⁺ cells by flow cytometry at pre-clinical (c), peak (d) and chronic (e) stages of EAE. Each number represents a mouse and shown is a representative of 3 (pre-clinical), 4 (peak) and 2 (chronic) experiments. Lines represent the mean of each group.