Figure 6
Antigen presentation function is enhanced in LMP2A transgenic mice when compared to litter mate controls.
a, Splenocytes from LMP2A and litter mate control mice (WT) were cultured with different concentrations of self-quenching DQ-OVA and FITC signal was measured from CD19+ cells cultured at 37°C (solid lines) and 4°C (dotted lines). b-d, 3H-thymidine uptake by T cells purified from TCRMOG mice and B cells purified from LMP2A-BCRMOG (LMP2A) and WT-BCRMOG (WT) mice were measured after culturing with indicated concentrations of rMOG or MOG35–55 peptide. Cells were cultured for three (b) or four (c and d) days and pulsed with 3H-thymidine for the final 24 hours. Before culturing, B cells were irradiated (b and c) or fixed with paraformaldehyde (d). The data are representative of 2 (a), 5 (b), 3 (c) and 3 (d) independent experiments. Vertical bars represent mean ± SD between triplicate wells. Statistical differences of p<0.05 (*) or p<0.01 (**) are indicated.
