Figure 2
From: Metformin kills and radiosensitizes cancer cells and preferentially kills cancer stem cells
Western blotting for the effect of metformin, 4 Gy irradiation or combined on the signals associated with AMPK/mTOR pathway in MCF-7 and FSaII cells.
Whole cell lysates were prepared and Western blot analysis was conducted using anti-p-AMPK, -AMPK, -p-ACC, -ACC, -p-mTOR,-mTOR, -p-S6K1, -S6K1, -p-4EBP1 and -4EBP1 antibodies. Experiments were repeated 4-5 times and the representative results are shown. The numbers under each blot are intensity of the blot relative to that of untreated control.
