Figure 5
From: Calcineurin Controls Voltage-Dependent-Inactivation (VDI) of the Normal and Timothy Cardiac Channels
Cyclosporine A effectively restores VDI of Timothy channel G406R.
(a) Normalized ICa (n = 10) (left) and IBa (n = 11) (right) records from representative oocytes expressing Cav1.2/G406R, in the presence or in the absence of 1 µM CsA. (b) Steady-state inactivation of Cav1.2G406R in the presence of increasing CsA concentrations, as indicated for ICa (left) and IBa (right). (c) Efficacy of CsA on VDI. Normalized maximal steady-state inactivating ICa at +10 mV (left) and IBa at 0 mV (right) plotted at increasing CsA concentrations. Data displayed as mean ± S.E.M (n = 8−12). Peak currents for ICa α11.2/G406R, −377 ± 40 nA (n = 13); α11.2/G406R + 50 nM CsA, −229 ± 43 nA (n = 11); IBa, α11.2/G406R, −377 ± 61 nA (n = 12); α11.2/G406R + 50 nM CsA, −194 ± 38 nA (n = 10).
