Figure 2

Hair follicle regeneration via intracutaneous transplantation of regenerated bioengineered hair follicles.

(a) Macroscopic observations of the transplantation procedure of the regenerated hair follicles. A slit is made using a surgical knife (left), the regenerated hair follicle is transplanted using forceps (middle) and a surgical tape bandage is used to cover the transplanted hair follicle (right). (b) Macroscopic observations of a transplanted regenerated hair follicle at day 16 (left panels) and day 22 (right panels). (c) Histological analyses of the transplanted regenerated hair follicles. H&E-staining (upper panels) and fluorescent microscopy (lower panels) of the transplanted regenerated hair follicle of a serial section is shown. The boxed areas in the left panels are shown at a higher magnification in the right panels. Broken lines indicate the outermost of the hair follicle. (d) Immunohistochemical analysis of the bulge region in a transplanted regenerated hair follicle. The bulge region was analyzed by immunostaining with specific antibodies for CD34 (upper center) and CD49f (upper right) and the sebaseous gland which was located upper region of the bulge was stained with oil red (upper left). The hair bulb was analyzed by immunostaining with anti-versican (lower left) and α-SMA (arrowheads, lower right) antibodies and by enzymatic staining for ALP (lower center). The nuclei were stained using Hoechst 33258 (Nuc, blue). The arrowheads show positively staining areas.