Figure 2

Primary tumors in Rb^ΔEC; p53^ΔEC and p53^ΔEC mice undergo EMT.

(a-b) Hierarchical clustering of mouse tumors and normal skin showing the discrimination between genotypes (a) and according to an EMT gene signature (b). Note that metastatic tumours (denoted by arrows) cluster together only in the second case. c-g’) Examples of spontaneous squamous (c, d, e, f, g) and spindle cell carcinoma (c’, d’, e’, f’, g’) showing the expression of E-Cadherin (c, c’), Vimentin (d, d’), Twist (e, e’), Snail (f, f’) and FoxC2 (g, g’). Dashed lines in c-g denote the boundaries between differentiated and undifferentiated areas in squamous tumours. Bars = 150 μm. (h) Western blot showing the expression of the quoted EMT markers and modulators in skin, squamous and spindle cell carcinomas. i) qRT-PCR analysis showing the expression of Snai1 gene in tumours according the mouse genotype (upper panel), metastatic behaviour (middle Panel) and tumour differentiation characteristics (lower panel) n = number of tumours analysed.