Figure 6

Flow cytometric analysis of B cell subsets in transport of prion rods.

Adoptive transfer experiment was performed similarly as experiments in Figure 5. Prion rods were injected IP into donor mice and six hours later immune cells harvested from the PC were washed and transferred into the PC of recipient mice. MedLN from donor (panels a–e) and recipient mice (panels f–h) were analyzed 6 HPI for prion-bearing B1 and B2 cells. Donor MedLN cells were analyzed for SSC^loCD21⁺B220⁺CD5⁻CD11b-CD23⁺ follicular B2 cells (b and d) and SSC^loB220⁻ CD21⁻CD23⁻CD11b⁺CD5⁺ B-1 cells (c and e) bearing prion rods. Recipient MedLNs were also analyzed for prion uptake by B2 cells (red peak (g) and dots (h)) and B1 cells (green). B cell subsets were compared to cells in MedLN from PBS inoculated control mice (blue). Cell counts in graphs are shown as log₁₀ per 10⁵ total cells (d and e) or per 10⁴ B cells (h).