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Figure 1

From: Human chondrogenic paraxial mesoderm, directed specification and prospective isolation from pluripotent stem cells

Figure 1

Directed specification of paraxial mesoderm in a chemically defined medium.

(A) Differentiation protocol for hPS cells. (B, C) GSK3 inhibitor and Noggin-dependent specification of paraxial mesoderm and associated changes in the progenitor profile after 8 days of differentiation of H9 hES cells. (B) FACS analysis. (C) RT-PCR with MEOX1 and TCF15 primers. No, None; N, Noggin; AceBIO/Ace-BIO, Acetoxime-BIO; CHIR, CHIR99021; MeBIO, Methyl-BIO. (D) Time-dependent differentiation of H9 hES cells in the presence of no factors (No), BIO and BIO + Noggin (BION). Specification of mesoderm was monitored by RT-PCR.

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