Figure 4

Factor-dependent chondrogenesis from the KDR⁻PDGFRα⁺ paraxial mesoderm-like cells from various hPS cell lines.

(A) Chondrogenesis from the H9 hES cell-derived KDR⁻PDGFRα⁺ paraxial mesoderm performed under P (a), PT (c), PB (d) and PTB (d) conditions in 2D micromass culture for 16 days. (Left) Period of treatment with PDGF (P, red), TGFβ3 (T, blue) and/or BMP4 (B, green) is indicated as a bar. (Right) The resulting micromasses were stained with acid Alcian Blue. Arrows indicate small cartilage nodules. Scale bars, 500 μm. (B) Expression of chondrogenesis-specific genes during 2D micromass culture of the H9 hES cell-derived KDR⁻PDGFRα⁻ paraxial mesodermal cells performed under P, PT, PB or PTB conditions. The resulting micromasses were subjected to RT-PCR on the day indicated with primers for the genes indicated.